Journal: Nature Biomedical Engineering

Article Title: CAR T cells expressing a bacterial virulence factor trigger potent bystander antitumour responses in solid cancers

doi: 10.1038/s41551-022-00875-5

Figure Lengend Snippet: a , Retroviral constructs used for mouse T-cell engineering. b , c , Treatment schedules for CAR-engineered T cells of subcutaneous murine A20 lymphoma that endogenously express murine CD19 ( b ), and murine NXS2 neuroblastoma that endogenously express GD2 ( c ). d , e , A20 tumour size (mean) until the first mouse had to be killed ( d ) and mouse survival (Kaplan–Meier curve) after treatment ( e ); arrows in e indicate rechallenge of cured mice with A20 tumour cells. f , g , NXS2 tumour size (mean) until the first mouse had to be killed ( f ) and mouse survival (Kaplan–Meier curve) after treatment ( g ). The A20 experiment was performed once, and the NXS2 experiment was performed twice and all data points were pooled. Tumour sizes between treatment groups were compared using two-way ANOVA, and survival curves were compared using the log-rank test. Error bars represent s.e.m. (NS, no statistical significance; * P < 0.05, *** P < 0.001, **** P < 0.0001). Precise P values are reported in Supplementary Table .

Article Snippet: Murine stem cell virus-based retroviral vector, pMIG-W (Luk Parijs, Addgene, plasmid 12282) was used as retroviral vector backbone to construct RV(CD19CAR) and RV(NAP-CD19CAR).

Techniques: Retroviral, Construct